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Introduction to RNA-Seq Analysis
March 11 @ 9:00 am - 12:00 pm
One event on March 11, 2022 at 9:00 am
Gene expression is central to cell biology. Disease pathways often involve changes in the expression levels of at least some genes. RNA-seq has become one of the most popular experimental methods to quantify gene expression levels. This 2-day hands-on workshop will provide an introduction to a typical bulk RNA-seq protocol and focus on the data analysis steps for recovering actionable insights.
You’ll learn how to perform quality checks of the raw data in the typical format provided by sequencing centers, process it by trimming adapters, map trimmed reads to a reference genome, and tally gene-wise counts. You’ll learn to analyze high-throughput data using a webtool and using a high-performance compute (HPC) cluster, learn about various methods of quality control at each step, and interpret data formats such as FASTQ, BAM, and GFF. No prior RNA-seq experience is required.
NOTE: Attendance at both sessions is required.
Before the workshop, create an account on the Galaxy platform.
This is an introductory workshop. No prior experience is required. No prerequisites.